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KMID : 0613820120220121718
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Journal of Life Science
2012 Volume.22 No. 12 p.1718 ~ p.1723
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Gibberellin A4 Producted by Fusarium solani Isolated from the Roots of Suaeda japonica Makino
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Seo Yeong-gyo
You Young-Hyun
Yoon Hyeok-Jun
Kang Sang-Mo
Kim Hyun
Kim Mi-ae
Kim Chang-Mu
Lee In-Jung
Kim Jong-Guk
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Abstract
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Ten endophytic fungi with different colony morphologies were isolated from the roots of Suaeda japonica Makino growing naturally in Suncheon Bay. Plant growth promotion was verified by treating waito-c rice seedlings with culture filtrates from the endophytic fungi. The bioassays showed that the Sj/7/4 fungal strain induced effective growth promotion in the seedlings. The gibberellins (GA) produced by fungal strain Sj/7/4 were analyzed by gas chromatography /mass spectroscopy with selected ion monitoring (GC/MS SIM). The culture filtrate of Sj/7/4 fungal strain was confirmed to contain GA₄ through quantitative analysis. The Sj/7/4 fungal strain was identified to determine the internal transcribed spacer (ITS) regions with universal primers ITS-1 and ITS-4 by using polymerase chain reactions (PCR). Molecular analysis of the Sj/7/4 fungal strain showed high similarity to Fusarium solani. The Sj/7/4 fungal strain isolated from the root of S. japonica was therefore designated as F. solani Sj/7/4.
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KEYWORD
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Fusarium solani, Suaeda japonica Makino, gibberellin, endophytic fungus
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